This investigation was to determine the changes in the trypsin inhibitor activity(TIA) and electrophoresis patterns of the soybean cotyledon and axis during germination.
The TIA of the cotyledon decreased slightly and that of the axis decreased rapidly to 50% activivity after 7 day germination. At the 2nd, 3rd and 4th day´s germination the TIA of the defatted dry axis was higher than that of cotyledon. However, the TIA of the fresh cotyledon was lower than that of the axis, due to its higher moisture content.
Results from the electrophoretic studies showed that band 1 (polymer, 15S etc.), 2 (11S), and 3(7S) whichare the major reserve proteins of soybean were decreased consid erably in cotyledon and axis and the fragments with Rm values between 0.5 and 1.0 were increased and band 5 showed up during germination. The band 4 of the cotyledon and band 6 of axis were not changed during germination. Generally speaking, the TIA and thereserve protein decreased as germination proceed.
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